ProSci

PPP1R3A Antibody

Product Code:
 
PSI-55-330
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-55-330-400ul400ul£626.00
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Further Information

Additional Names:
Protein phosphatase 1 regulatory subunit 3A, Protein phosphatase 1 glycogen-associated regulatory subunit, Protein phosphatase type-1 glycogen targeting subunit, RG1, PPP1R3A, PP1G
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase. [provided by RefSeq].
Background References:
  • Yoshida, T., et al. Int. J. Mol. Med. 24(2):233-246(2009)
  • Yoshida, T., et al. Genomics 93(3):221-226(2009)
  • Gieger, C., et al. PLoS Genet.(11), E1000282 (2008) :
  • Savage, D.B., et al. PLoS Med.(1), E27 (2008) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This PPP1R3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-782 amino acids from the Central region of human PPP1R3A.
NCBI Gene ID #:
5506
NCBI Official Name:
Protein phosphatase 1 regulatory subunit 3A
NCBI Official Symbol:
PPP1R3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
126 kDa
Protein Accession #:
Q16821
Protein GI Number:
298286906
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q16821
User NOte:
Optimal dilutions for each application to be determined by the researcher.