ProSci

PDHA2 Antibody

Product Code:
 
PSI-55-394
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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PDHA2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
PDHA2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-394-400ul400ul£626.00
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Further Information

Additional Names:
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial, PDHE1-A type II, PDHA2, PDHAL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Background References:
  • Pinheiro, A., et al. Mol. Genet. Metab. 99(4):425-430(2010)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Caruso, M., et al. J. Biol. Chem. 276(48):45088-45097(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PDHA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the Central region of human PDHA2.
NCBI Gene ID #:
5161
NCBI Official Name:
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
NCBI Official Symbol:
PDHA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
P29803
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P29803
User NOte:
Optimal dilutions for each application to be determined by the researcher.