ProSci

FMO3 Antibody

Product Code:
 
PSI-62-528
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of FMO3 Antibody in MDA-MB231 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with FMO3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
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Western blot analysis of FMO3 Antibody in MDA-MB231 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with FMO3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-528-400ul400ul£626.00
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Further Information

Additional Names:
Dimethylaniline monooxygenase [N-oxide-forming] 3, Dimethylaniline oxidase 3, FMO II, FMO form 2, Hepatic flavin-containing monooxygenase 3, FMO 3, Trimethylamine monooxygenase, FMO3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
FMO3 is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. It plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds.
Background References:
  • Allerston,C.K., et.al., Mol. Genet. Metab. 98 (1-2), 198-202 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FMO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-56 amino acids from the N-terminal region of human FMO3.
NCBI Gene ID #:
2328
NCBI Official Name:
Dimethylaniline monooxygenase [N-oxide-forming] 3
NCBI Official Symbol:
FMO3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
P31513
Protein GI Number:
6166183
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P31513
User NOte:
Optimal dilutions for each application to be determined by the researcher.