ProSci

OTC Antibody

Product Code:
 
PSI-62-542
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 4
Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis in SW620 cell line and mouse liver, rat liver lysates (35ug/lane).
3 / 4
4 / 4
Flow cytometric analysis of ATDC5 cells using OTC Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in SW620 cell line and mouse liver, rat liver lysates (35ug/lane).
Flow cytometric analysis of ATDC5 cells using OTC Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-542-400ul400ul£662.00
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Further Information

Additional Names:
Ornithine carbamoyltransferase, mitochondrial, Ornithine transcarbamylase, OTCase, OTC
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
OTC is a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia.
Background References:
  • Hansmannel,F., et.al., Neurosci. Lett. 449 (1), 76-80 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This OTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-98 amino acids from the Central region of human OTC.
NCBI Gene ID #:
5009
NCBI Official Name:
Ornithine carbamoyltransferase, mitochondrial
NCBI Official Symbol:
OTC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
P00480
Protein GI Number:
84028235
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P00480
User NOte:
Optimal dilutions for each application to be determined by the researcher.