Further Information
Dual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, Mitogen-activated protein kinase phosphatase 1, MAP kinase phosphatase 1, MKP-1, Protein-tyrosine phosphatase CL100, DUSP1, CL100, MKP1, PTPN10, VH1
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has
intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
- Wu,W., et al., J. Biol. Chem. 280 (6), 4117-4124 (2005).
- Small,G.W., et al., Mol. Pharmacol. 66 (6), 1478-1490 (2004).
- Chandrasekharan,U.M., et al., J. Biol. Chem. 279 (45), 46678-46685 (2004).
- Mizuno,R., et al., J. Urol. 172 (2), 723-727 (2004).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Mouse
This DUSP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-76 amino acids from the N-terminal region of human DUSP1.
1843
Dual specificity protein phosphatase 1
DUSP1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
P28562
1346900
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Signal Transduction
P28562
Optimal dilutions for each application to be determined by the researcher.