ProSci

WNK3 Antibody

Product Code:
 
PSI-62-623
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis in uterus tumor cell line lysates (35ug/lane).
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WNK3(PRKWNK3) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in uterus tumor cell line lysates (35ug/lane).
WNK3(PRKWNK3) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-62-623-400ul400ul£626.00
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This product comes from: United States.
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Further Information

Additional Names:
Serine/threonine-protein kinase WNK3, Protein kinase lysine-deficient 3, Protein kinase with no lysine 3, WNK3, KIAA1566, PRKWNK3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Members of the 'with no lysine' (WNK) kinase family, such as WNK3, are serine-threonine protein kinases that lack the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site. Instead, these kinases have a conserved lysine in subdomain I that is thought to provide this function (Holden et al., 2004 [PubMed 15194194]).[supplied by OMIM]
Background References:
  • Verissimo, F., et al., Oncogene 20(39):5562-5569 (2001).
  • Nagase, T., et al., DNA Res. 7(4):273-281 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This WNK3 (PRKWNK3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1586-1616 amino acids from the C-terminal region of human WNK3 (PRKWNK3).
NCBI Gene ID #:
65267
NCBI Official Name:
Serine/threonine-protein kinase WNK3
NCBI Official Symbol:
WNK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
198 kDa
Protein Accession #:
Q9BYP7
Protein GI Number:
353526307
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9BYP7
User NOte:
Optimal dilutions for each application to be determined by the researcher.