ProSci

CCT8 Antibody

Product Code:
 
PSI-56-466
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in K562 cell line lysates (35ug/lane).
2 / 3
CCT8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in K562 cell line lysates (35ug/lane).
CCT8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-56-466-400ul400ul£626.00
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Further Information

Additional Names:
T-complex protein 1 subunit theta, TCP-1-theta, CCT-theta, Renal carcinoma antigen NY-REN-15, CCT8, C21orf112, CCTQ, KIAA0002
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Molecular chaperone; CCT8 assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Background References:
  • Mukherjee, K., et al. BMC Evol. Biol. 10, 64 (2010) :
  • Zebol, J.R., et al. Int. J. Biochem. Cell Biol. 41(4):822-827(2009)
  • Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
  • Hu, Y.H., et al. BMC Genomics 7, 155 (2006) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse
Immunogen:
This CCT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 amino acids from the C-terminal region of human CCT8.
NCBI Gene ID #:
10694
NCBI Official Name:
T-complex protein 1 subunit theta
NCBI Official Symbol:
CCT8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
P50990
Protein GI Number:
9988062
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P50990
User NOte:
Optimal dilutions for each application to be determined by the researcher.