ProSci

GLP1 Antibody

Product Code:
 
PSI-61-954
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in K562 cell line lysates (35ug/lane).
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-954-400ul400ul£626.00
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Further Information

Additional Names:
GATA-type zinc finger protein 1, GATA-like protein 1, GLP-1, ZGLP1, GLP1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Glucagon is initially synthesised as proglucagon, which is 180 amino acids in length and is cleaved proteolytically. It contains a region corresponding to Glucagon itself, in addition to a signal peptide, spacer regions and two Glucagon-like peptide sequences. GLP1 is encoded by the fourth exon of the six in the human Glucagon gene. It is 37 amino acids in length and has 48% amino acid sequence homology to Glucagon, and is thought to have arisen by gene duplication. The sequence of GLP1 is conserved across many species, however, indicating that it has a particular function. GLP1 is a potent insulin secretagogue, renders pancreatic beta cells 'glucose-competent' and may be useful in the treatment of diabetes mellitus.
Background References:
  • Sanz, C., et al. Am. J. Physiol. Endocrinol. Metab. 298 (3), E634-E643 (2010) :
  • Brown, R.J., et al. Diabetes Care 32(12):2184-2186(2009)
  • Li, S., et al. Dev. Biol. 301(1):106-116(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GLP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-112 amino acids from the Central region of human GLP1.
NCBI Gene ID #:
100125288
NCBI Official Name:
GATA-type zinc finger protein 1
NCBI Official Symbol:
ZGLP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
P0C6A0
Protein GI Number:
166990464
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P0C6A0
User NOte:
Optimal dilutions for each application to be determined by the researcher.