ProSci

MGAT2 Antibody

Product Code:
 
PSI-61-381
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MCF-7, HepG2 cell line lysates (35ug/lane).
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Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in MCF-7, HepG2 cell line lysates (35ug/lane).
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-381-400ul400ul£626.00
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Further Information

Additional Names:
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Beta-1,2-N-acetylglucosaminyltransferase II, GlcNAc-T II, GNT-II, Mannoside acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, MGAT2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
MGAT2 (UDP-N-acetylglucosamine:alpha-6-D-mannoside-beta-1, 2-N-acetylglucosaminyltransferase II) is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in MGAT2 may lead to carbohydrate-deficient glycoprotein syndrome, type II.
Background References:
  • Yen, C.L., et al., J. Biol. Chem. 278(20):18532-18537 (2003). Chen, S.H., et al., Glycoconj. J. 15(3):301-308 (1998). Tan, J., et al., Am. J. Hum. Genet. 59(4):810-817 (1996). Tan, J., et al., Eur. J. Biochem. 231(2):317-328 (1995). D'Agostaro, G.A., et al., J. Biol. Chem. 270(25):15211-15221 (1995).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat
Immunogen:
This MGAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-439 amino acids from the C-terminal region of human MGAT2.
NCBI Gene ID #:
4247
NCBI Official Name:
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
NCBI Official Symbol:
MGAT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
Q10469
Protein GI Number:
1708004
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q10469
User NOte:
Optimal dilutions for each application to be determined by the researcher.