Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Fluorescent confocal image of Hela cell stained with MBD2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBD2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MBD2 immunoreactivity is localized to Nucleus significantly. Fluorescent confocal image of Hela cell stained with MBD2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBD2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MBD2 immunoreactivity is localized to Nucleus significantly.](https://www.prosci-inc.com/static-images/60-764_1.jpg)
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![Western blot analysis in HL-60 cell line lysates (35ug/lane). Western blot analysis in HL-60 cell line lysates (35ug/lane).](https://www.prosci-inc.com/static-images/60-764_2.jpg)
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This product comes from:
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Typical lead time:
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- Further Information
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Further Information
Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
DNA methylation is the major modification of eukaryotic
genomes and plays an essential role in mammalian development. Human
proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
nuclear proteins related by the presence in each of a methyl-CpG
binding domain (MBD). Each of these proteins, with the exception of
MBD3, is capable of binding specifically to methylated DNA. MECP2,
MBD1 and MBD2 can also repress transcription from methylated gene
promoters. The protein encoded by this gene may function as a
mediator of the biological consequences of the methylation signal.
It is also reported that the this protein functions as a
demethylase to activate transcription, as DNA methylation causes
gene silencing.
- Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
- Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
- Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009)
- McDonough, C.W., et al. Hum. Genet. (2009) In press :
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Mouse
This MBD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human MBD2.
8932
Methyl-CpG-binding domain protein 2
MBD2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Q9UBB5
50401198
This antibody is purified through a protein A column, followed by peptide affinity purification.
Q9UBB5
Optimal dilutions for each application to be determined by the researcher.