ProSci

HMGB3 Antibody

Product Code:
 
PSI-57-896
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 2
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

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PSI-57-896-400ul400ul£626.00
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Further Information

Additional Names:
High mobility group protein B3, High mobility group protein 2a, HMG-2a, High mobility group protein 4, HMG-4, HMGB3, HMG2A, HMG4
Application Note:
For WB starting dilution is: 1:1000
Background:
HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).
Background References:
  • Nemeth, M.J., et al. Proc. Natl. Acad. Sci. U.S.A. 103(37):13783-13788(2006)
  • Ross, M.T., et al. Nature 434(7031):325-337(2005)
  • Vaccari, T., et al. Genomics 49(2):247-252(1998)
  • Wilke, K., et al. Gene 198 (1-2), 269-274 (1997) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse
Immunogen:
This HMGB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the Central region of human HMGB3.
NCBI Gene ID #:
3149
NCBI Official Name:
High mobility group protein B3
NCBI Official Symbol:
HMGB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
O15347
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O15347
User NOte:
Optimal dilutions for each application to be determined by the researcher.