ProSci

PAPOLG Antibody

Product Code:
 
PSI-56-905
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

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PSI-56-905-400ul400ul£626.00
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Further Information

Additional Names:
Poly(A) polymerase gamma, PAP-gamma, Neo-poly(A) polymerase, Neo-PAP, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme, PAPOLG, PAP2, PAPG
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14.
Background References:
  • Lee, Y.S., et al. J. Biol. Chem. 285(36):28105-28116(2010)
  • Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
  • Kyriakopoulou, C.B., et al. J. Biol. Chem. 276(36):33504-33511(2001)
  • Topalian, S.L., et al. Mol. Cell. Biol. 21(16):5614-5623(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PAPOLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 685-714 amino acids from the C-terminal region of human PAPOLG.
NCBI Gene ID #:
64895
NCBI Official Name:
Poly(A) polymerase gamma
NCBI Official Symbol:
PAPOLG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
83 kDa
Protein Accession #:
Q9BWT3
Protein GI Number:
116242699
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9BWT3
User NOte:
Optimal dilutions for each application to be determined by the researcher.