ProSci

GTD2A Antibody

Product Code:
 
PSI-61-726
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of GTD2A Antibody in K562 cell line lysates (35ug/lane)
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of GTD2A Antibody in K562 cell line lysates (35ug/lane)
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-726-400ul400ul£626.00
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Further Information

Additional Names:
General transcription factor II-I repeat domain-containing protein 2A, GTF2I repeat domain-containing protein 2A, Transcription factor GTF2IRD2-alpha, GTF2IRD2, GTF2IRD2A
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
GTD2A is unusual in that its coding sequence is mostly derived from Charlie8 repeat elements. However, there is mRNA and EST evidence to suggest that this gene is transcribed, and the encoded protein has a homolog in mouse, with which it shares 78% sequence identity. The exact function of this gene product is not known. It is inferred to be a transcription factor based on the presence of GTF2I-like repeats (containing helix-loop-helix motifs), also found in other proteins such as GTF2IRD1 and GTF2I. These three genes are clustered together on chr 7q11.23.
Background References:
  • Hinsley, T.A., et al. Protein Sci. 13(10):2588-2599(2004)
  • Makeyev, A.V., et al. Proc. Natl. Acad. Sci. U.S.A. 101(30):11052-11057(2004)
  • Tipney, H.J., et al. Eur. J. Hum. Genet. 12(7):551-560(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GTD2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 812-841 amino acids from the C-terminal region of human GTD2A.
NCBI Gene ID #:
84163
NCBI Official Name:
General transcription factor II-I repeat domain-containing protein 2A
NCBI Official Symbol:
GTF2IRD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
107 kDa
Protein Accession #:
Q86UP8
Protein GI Number:
187607027
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q86UP8
User NOte:
Optimal dilutions for each application to be determined by the researcher.