ProSci

ELP3 Antibody

Product Code:
 
PSI-55-654
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis of ELP3 using rabbit polyclonal ELP3 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ELP3 gene (Lane 2).
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Formalin-fixed and paraffin-embedded human testis tissue reacted with ELP3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of ELP3 using rabbit polyclonal ELP3 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ELP3 gene (Lane 2).
Formalin-fixed and paraffin-embedded human testis tissue reacted with ELP3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-55-654-400ul400ul£626.00
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Further Information

Additional Names:
Elongator complex protein 3, hELP3, ELP3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Elongator complex protein 3 (ELP3) is a catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. It may also have a methyltransferase activity.
Background References:
  • Han,Q., Acta Biochim. Biophys. Sin. (Shanghai) 39 (6), 453-461 (2007)
  • Kim,J.H., Proc. Natl. Acad. Sci. U.S.A. 99 (3), 1241-1246 (2002)
  • Ninomiya,Y., J. Biochem. 118 (2), 380-389 (1995)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ELP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 468-498 amino acids from the C-terminal region of human ELP3.
NCBI Gene ID #:
55140
NCBI Official Name:
Elongator complex protein 3
NCBI Official Symbol:
ELP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
Q9H9T3
Protein GI Number:
145558902
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9H9T3
User NOte:
Optimal dilutions for each application to be determined by the researcher.