ProSci

MARS Antibody

Product Code:
 
PSI-63-237
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of MARS Antibody in HL-60 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of MARS Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-237-400ul400ul£626.00
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Further Information

Additional Names:
Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase, MetRS, MARS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Methionyl-tRNA synthetase belongs to the class I family of tRNA synthetases. It catalyzes the chemical reaction: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Background References:
  • Kaminska,M., Biochemistry 40 (47), 14309-14316 (2001)
  • Kang,J., J. Biol. Chem. 275 (41), 31682-31688 (2000)
  • Ko,Y.G., J. Cell Biol. 149 (3), 567-574 (2000)
  • Quevillon,S., J. Mol. Biol. 285 (1), 183-195 (1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 872-899 amino acids from the C-terminal region of human MARS.
NCBI Gene ID #:
4141
NCBI Official Name:
Methionine--tRNA ligase, cytoplasmic
NCBI Official Symbol:
MARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
101 kDa
Protein Accession #:
P56192
Protein GI Number:
20178332
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P56192
User NOte:
Optimal dilutions for each application to be determined by the researcher.