ProSci

TCEA1 Antibody

Product Code:
 
PSI-57-969
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in Hela cell line lysates (35ug/lane).
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TCEA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Hela cell line lysates (35ug/lane).
TCEA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-57-969-400ul400ul£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
Transcription elongation factor A protein 1, Transcription elongation factor S-II protein 1, Transcription elongation factor TFIISo, TCEA1, GTF2S, TFIIS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Background References:
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TCEA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the Central region of human TCEA1.
NCBI Gene ID #:
6917
NCBI Official Name:
Transcription elongation factor A protein 1
NCBI Official Symbol:
TCEA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
P23193
Protein GI Number:
1174652
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P23193
User NOte:
Optimal dilutions for each application to be determined by the researcher.