ProSci

NCALD Antibody

Product Code:
 
PSI-55-219
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).

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PSI-55-219-400ul400ul£626.00
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Further Information

Additional Names:
Neurocalcin-delta, NCALD
Application Note:
For WB starting dilution is: 1:1000
Background:
NCALD encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010)
  • Gill, M.B., et al. J. Biol. Chem. 284(21):14503-14512(2009)
  • Venkataraman, V., et al. Biochemistry 47(25):6590-6601(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig, Rat, Chicken, Monkey, Xenopus
Immunogen:
This NCALD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-174 amino acids from the C-terminal region of human NCALD.
NCBI Gene ID #:
83988
NCBI Official Name:
Neurocalcin-delta
NCBI Official Symbol:
NCALD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
P61601
Protein GI Number:
47606440
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
P61601
User NOte:
Optimal dilutions for each application to be determined by the researcher.