ProSci

GJA10 Antibody

Product Code:
 
PSI-58-186
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of GJA10 Antibody in mouse heart tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of GJA10 Antibody in mouse heart tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

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PSI-58-186-400ul400ul£626.00
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Further Information

Additional Names:
Gap junction alpha-9 protein, Connexin-58, Cx58, Connexin-59, Cx59, Gap junction alpha-10 protein, GJA9, GJA10
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
GJA8 is a an integral membrane protein that belongs to the connexin family, alpha-type (group II) subfamily. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.
Background References:
  • Hombach S, et al., EurNeurosci. 2004 May;19(10):2633-40.
  • Houghton FD, et al., Biol Reprod. 2002 May;66(5):1403-12.
  • Manthey D, et al.,Biol Chem. 1999 May 21;274(21):14716-23.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GJA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-515 amino acids from the C-terminal region of human GJA10.
NCBI Gene ID #:
81025
NCBI Official Name:
Gap junction alpha-9 protein
NCBI Official Symbol:
GJA9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
P57773
Protein GI Number:
50403749
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P57773
User NOte:
Optimal dilutions for each application to be determined by the researcher.