ProSci

Mouse Pkmyt1 Antibody

Product Code:
 
PSI-57-464
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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Western blot analysis of Pkmyt1 using rabbit polyclonal Mouse Pkmyt1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pkmyt1 gene.
3 / 3
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western blot analysis of Pkmyt1 using rabbit polyclonal Mouse Pkmyt1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pkmyt1 gene.

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PSI-57-464-400ul400ul£626.00
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Further Information

Additional Names:
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, Pkmyt1, Myt1
Application Note:
For WB starting dilution is: 1:1000
Background:
Pkmyt1 acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Pkmyt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-143 amino acids from the N-terminal region of mouse Pkmyt1.
NCBI Gene ID #:
268930
NCBI Official Name:
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
NCBI Official Symbol:
Pkmyt1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
Q9ESG9
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9ESG9
User NOte:
Optimal dilutions for each application to be determined by the researcher.