ProSci

E2F2 Antibody

Product Code:
 
PSI-55-208
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 ug per lane.
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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).

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PSI-55-208-400ul400ul£626.00
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Further Information

Additional Names:
Transcription factor E2F2, E2F-2, E2F2
Application Note:
For WB starting dilution is: 1:1000
Background:
E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq].
Background References:
  • Revenko, A.S., et al. Mol. Cell. Biol. 30(22):5260-5272(2010)
  • Hayami, S., et al. Mol. Cancer 9, 59 (2010) :
  • Chen, J., et al. Cancer Causes Control 20(9):1769-1777(2009)
  • Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This E2F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the Central region of human E2F2.
NCBI Gene ID #:
1870
NCBI Official Name:
Transcription factor E2F2
NCBI Official Symbol:
E2F2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
Q14209
Protein GI Number:
2494228
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q14209
User NOte:
Optimal dilutions for each application to be determined by the researcher.