ProSci

GTF2B Antibody

Product Code:
 
PSI-57-669
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis of GTF2B using rabbit polyclonal GTF2B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2B gene.
2 / 2
Western blot analysis in mouse kidney tissue lysates (35ug/lane).
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Western blot analysis of GTF2B using rabbit polyclonal GTF2B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2B gene.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).

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PSI-57-669-400ul400ul£626.00
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Further Information

Additional Names:
Transcription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.
Background References:
  • Wang, Y., et al. Curr. Biol. 20(6):548-553(2010)
  • Thompson, N.E., et al. J. Biol. Chem. 284(37):24754-24766(2009)
  • Gilman, B., et al. J. Biol. Chem. 284(14):9093-9098(2009)
  • Vogt, C., et al. J. Virol. 82(22):11446-11453(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Rat
Immunogen:
This GTF2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human GTF2B.
NCBI Gene ID #:
2959
NCBI Official Name:
Transcription initiation factor IIB
NCBI Official Symbol:
GTF2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
Q00403
Protein GI Number:
135629
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q00403
User NOte:
Optimal dilutions for each application to be determined by the researcher.