ProSci

MTA1 Antibody

Product Code:
 
PSI-60-671
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A431 whole cell lysate Lane 4: mouse brain lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in 293 cell line lysates (35ug/lane).
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Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A431 whole cell lysate Lane 4: mouse brain lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293 cell line lysates (35ug/lane).

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PSI-60-671-400ul400ul£626.00
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Further Information

Additional Names:
Metastasis-associated protein MTA1, MTA1
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. [provided by RefSeq].
Background References:
  • Zhu, X., et al.Thorac Oncol 5(8):1159-1166(2010)
  • Van Rechem, C., et al. Mol. Cell. Biol. 30(16):4045-4059(2010)
  • Yang, Y.M., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 26(7):682-684(2010)
  • Li, D.Q., et al. J. Biol. Chem. 285(26):19802-19812(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This MTA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 659-687 amino acids from the C-terminal region of human MTA1.
NCBI Gene ID #:
9112
NCBI Official Name:
Metastasis-associated protein MTA1
NCBI Official Symbol:
MTA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
81 kDa
Protein Accession #:
Q13330
Protein GI Number:
259016275
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q13330
User NOte:
Optimal dilutions for each application to be determined by the researcher.