ProSci

ST8SIA4 Antibody

Product Code:
 
PSI-64-105
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of ST8SIA4 Antibody in HL-60 cell line lysates (35ug/lane) Western blot analysis of ST8SIA4 Antibody in mouse spleen tissue lysates (35ug/lane)
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ST8SIA4 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of ST8SIA4 Antibody in HL-60 cell line lysates (35ug/lane) Western blot analysis of ST8SIA4 Antibody in mouse spleen tissue lysates (35ug/lane)
ST8SIA4 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-64-105-400ul400ul£626.00
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Further Information

Additional Names:
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase, 2499-, Alpha-2,8-sialyltransferase 8D, Polysialyltransferase-1, Sialyltransferase 8D, SIAT8-D, Sialyltransferase St8Sia IV, ST8SiaIV, ST8SIA4, PST, PST1, SIAT8D
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein, which is a member of glycosyltransferase family 29, is a type II membrane protein that may be present in the Golgi apparatus.
Background References:
  • Johnson, M.P., et al. Hum. Genet. 126(5):655-666(2009)
  • Foley, D.A., et al. J. Biol. Chem. 284(23):15505-15516(2009)
  • Otowa, T., et al. J. Hum. Genet. 54(2):122-126(2009)
  • Sonuga-Barke, E.J., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 147B (8), 1359-1368 (2008) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ST8SIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-214 amino acids from the Central region of human ST8SIA4.
NCBI Gene ID #:
7903
NCBI Official Name:
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase
NCBI Official Symbol:
ST8SIA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
Q92187
Protein GI Number:
2494834
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q92187
User NOte:
Optimal dilutions for each application to be determined by the researcher.