ProSci

GEN1 Antibody

Product Code:
 
PSI-63-976
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of lysate from MDA-MB-453 cell line, using GEN1 Antibody at 1:1000.
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Western blot analysis of GEN1 Antibody in mouse liver tissue lysates (35ug/lane)
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Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of lysate from MDA-MB-453 cell line, using GEN1 Antibody at 1:1000.
Western blot analysis of GEN1 Antibody in mouse liver tissue lysates (35ug/lane)

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PSI-63-976-400ul400ul£626.00
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Further Information

Additional Names:
Flap endonuclease GEN homolog 1, 31--, GEN1
Application Note:
For WB starting dilution is: 1:1000
Background:
Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded (By similarity).
Background References:
  • Ip, S.C., et al. Nature 456(7220):357-361(2008)
  • Ishikawa, G., et al. Nucleic Acids Res. 32(21):6251-6259(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human GEN1.
NCBI Gene ID #:
348654
NCBI Official Name:
Flap endonuclease GEN homolog 1
NCBI Official Symbol:
GEN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
103 kDa
Protein Accession #:
Q17RS7
Protein GI Number:
290457644
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q17RS7
User NOte:
Optimal dilutions for each application to be determined by the researcher.