Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Western Blot at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane. Western Blot at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.](https://www.prosci-inc.com/static-images/63-692_1.jpg)
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![Western blot analysis in 293,293T cell line ,mouse liver and rat liver tissue lysates (35ug/lane). Western blot analysis in 293,293T cell line ,mouse liver and rat liver tissue lysates (35ug/lane).](https://www.prosci-inc.com/static-images/63-692_2.jpg)
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![](https://www.prosci-inc.com/static-images/63-692_3.jpg)
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![Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.](https://www.prosci-inc.com/static-images/63-692_4.jpg)
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![Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](https://www.prosci-inc.com/static-images/63-692_5.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
Ornithine aminotransferase, mitochondrial, Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase, Ornithine aminotransferase, hepatic form, Ornithine aminotransferase, renal form, OAT
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
OAT is the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA.
- Michaud J., et.al., Am. J. Hum. Genet. 56:616-622(1995).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This OAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human OAT.
4942
Ornithine aminotransferase, mitochondrial
OAT
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
P04181
129018
This antibody is purified through a protein A column, followed by peptide affinity purification.
Obesity,Neuroscience,Signal Transduction
P04181
Optimal dilutions for each application to be determined by the researcher.