Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Western blot analysis in A549,HepG2,NCI-H292 cell line,mouse liver,lung and rat liver tissue lysates (35ug/lane). Western blot analysis in A549,HepG2,NCI-H292 cell line,mouse liver,lung and rat liver tissue lysates (35ug/lane).](https://www.prosci-inc.com/static-images/57-649_1.jpg)
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![Fluorescent confocal image of A549 cell stained with ALDH2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ALDH2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ALDH2 immunoreactivity is localized to Mitochondrion significantly. Fluorescent confocal image of A549 cell stained with ALDH2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ALDH2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ALDH2 immunoreactivity is localized to Mitochondrion significantly.](https://www.prosci-inc.com/static-images/57-649_2.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
Aldehyde dehydrogenase, mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDH2, ALDM
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Asians have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Asians than among Caucasians could be related to the absence of the mitochondrial isozyme.
- Guo,Y.M., World J. Gastroenterol. 14 (9), 1444-1449 (2008) Chen,L., PLoS Med.(3), E52 (2008) Teeguarden,J.G., Inhal Toxicol 20 (4), 375-390 (2008) Yoshida,A., Pharmacogenetics(4), 139-147 (1992)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This ALDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human ALDH2.
217
Aldehyde dehydrogenase, mitochondrial
ALDH2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
P05091
118504
This antibody is purified through a protein A column, followed by peptide affinity purification.
Obesity,Signal Transduction
P05091
Optimal dilutions for each application to be determined by the researcher.