ProSci

Vinculin

Product Code:
 
PSI-61-326
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: mouse kidney lysate Lane 5: PC-12 whole cell lysate Lane 6: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: mouse kidney lysate Lane 5: PC-12 whole cell lysate Lane 6: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

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PSI-61-326-200ul200ul£626.00
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Further Information

Additional Names:
Vinculin, Metavinculin, Vcl
Application Note:
For WB starting dilution is: 1:2000
Background:
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).
Background References:
  • Coll J.-L.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:9161-9165(1995).
  • Alatortsev V.E.,et al.FEBS Lett. 413:197-201(1997).
  • Carninci P.,et al.Science 309:1559-1563(2005).
  • Lubec G.,et al.Submitted (JAN-2009) to UniProtKB.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 903-937 amino acids from mouse.
NCBI Gene ID #:
22330
NCBI Official Name:
Vinculin
NCBI Official Symbol:
Vcl
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
117 kDa
Protein Accession #:
Q64727
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q64727
User NOte:
Optimal dilutions for each application to be determined by the researcher.