ProSci

COL9A1 Antibody

Product Code:
 
PSI-61-492
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in MCF-7 cell line and mouse eyeball,rat eyeball tissue lysates (35ug/lane).
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Western Blot at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MCF-7 cell line and mouse eyeball,rat eyeball tissue lysates (35ug/lane).

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PSI-61-492-400ul400ul£626.00
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Further Information

Additional Names:
Collagen alpha-1(IX) chain, COL9A1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
COL9A1 is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule,and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in the COL9A1 gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects.
Background References:
  • Fresquet,M., J. Biol. Chem. 282 (48), 34634-34643 (2007)
  • Liu,L.Y., Yi Chuan 29 (4), 427-432 (2007)
  • Van Camp,G., Am. J. Hum. Genet. 79 (3), 449-457 (2006)
  • Sivakumaran,T.A., J. Assoc. Res. Otolaryngol.(2), 160-172 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This COL9A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-456 amino acids from the Central region of human COL9A1.
NCBI Gene ID #:
1297
NCBI Official Name:
Collagen alpha-1(IX) chain
NCBI Official Symbol:
COL9A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
92 kDa
Protein Accession #:
P20849
Protein GI Number:
296439373
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P20849
User NOte:
Optimal dilutions for each application to be determined by the researcher.