Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Western blot analysis of lysates from SH-SY5Y cell line and mouse brain tissue (from left to right),using UCHL1 Antibody .AP2126e was diluted at 1:1000 at each lane. Western blot analysis of lysates from SH-SY5Y cell line and mouse brain tissue (from left to right),using UCHL1 Antibody .AP2126e was diluted at 1:1000 at each lane.](https://www.prosci-inc.com/static-images/61-088_1.jpg)
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![Western blot analysis in U266,NCI-H1299,mouse Neuro-2a cell line and mouse brain tissue lysates (35ug/lane). Western blot analysis in U266,NCI-H1299,mouse Neuro-2a cell line and mouse brain tissue lysates (35ug/lane).](https://www.prosci-inc.com/static-images/61-088_2.jpg)
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![](https://www.prosci-inc.com/static-images/61-088_3.jpg)
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![UCHL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. UCHL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.](https://www.prosci-inc.com/static-images/61-088_4.jpg)
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![UCHL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. UCHL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.](https://www.prosci-inc.com/static-images/61-088_5.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).
- Maraganore, D.M., et al., Mov Disord 18(6):631-636 (2003).
- Nishikawa, K., et al., Biochem. Biophys. Res. Commun. 304(1):176-183 (2003).
- Liu, Y., et al., Cell 111(2):209-218 (2002).
- Caballero, O.L., et al., Oncogene 21(19):3003-3010 (2002).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Mouse, Monkey, Pig, Bovine
This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-214 amino acids from the C-terminal region of human UCHL1.
7345
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UCHL1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
P09936
136681
This antibody is purified through a protein A column, followed by peptide affinity purification.
Cell Cycle,Neuroscience
P09936
Optimal dilutions for each application to be determined by the researcher.