Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.](https://www.prosci-inc.com/static-images/61-046_1.jpg)
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![Flow cytometric analysis of MCF-7 cells using ADRA1D Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. Flow cytometric analysis of MCF-7 cells using ADRA1D Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.](https://www.prosci-inc.com/static-images/61-046_2.jpg)
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![](https://www.prosci-inc.com/static-images/61-046_3.jpg)
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![Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.](https://www.prosci-inc.com/static-images/61-046_4.jpg)
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![Western blot analysis of lysates from A549, LNCaP, PC-3 cell line and rat brain tissue lysate (from left to right), using ADRA1D Antibody at 1:1000 at each lane. Western blot analysis of lysates from A549, LNCaP, PC-3 cell line and rat brain tissue lysate (from left to right), using ADRA1D Antibody at 1:1000 at each lane.](https://www.prosci-inc.com/static-images/61-046_5.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor, Alpha-adrenergic receptor 1a, ADRA1D, ADRA1A
For IHC-P starting dilution is: 1:25
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000
This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
- Bruno J.F.,et al.Biochem. Biophys. Res. Commun. 179:1485-1490(1991).
- Forray C.,et al.Mol. Pharmacol. 45:703-708(1994).
- Schwinn D.A.,et al.J. Pharmacol. Exp. Ther. 272:134-142(1995).
- Weinberg D.H.,et al.Biochem. Biophys. Res. Commun. 201:1296-1304(1994).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Mouse, Bovine, Dog, Rabbit, Sheep
This ADRA1D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30amino acids from the N-terminal region of human ADRA1D.
146
Alpha-1D adrenergic receptor
ADRA1D
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
P25100
1168243
This antibody is purified through a protein A column, followed by peptide affinity purification.
Neuroscience,Signal Transduction,Growth Factor
P25100
Optimal dilutions for each application to be determined by the researcher.