APOB Antibody

Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Apolipoprotein B (ApoB) is a protein that in humans is encoded by the APOB gene. This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Amino acids Q246-N450 from the human protein were used as the immunogen for the APOB antibody. This sequence is common to both the full length (ApoB-100) and truncated (ApoB-48) forms of the protein.

This APOB antibody is available for research use only.

Cytoplasmic, membranous

Antigen affinity

Human

P04114