NSJ Bioreagents

APOBEC3A Antibody / PHO1 / Phorbolin 1

Product Code:
 
NSJ-RQ4184
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
After reconstitution, the APOBEC3A antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 2
Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2 and 4) mouse SP20 cell lysate with APOBEC3A antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa, observed here at ~28 kDa.
2 / 2
Flow cytometry testing of human A549 cells with APOBEC3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= APOBEC3A antibody.
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Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2 and 4) mouse SP20 cell lysate with APOBEC3A antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa, observed here at ~28 kDa.
Flow cytometry testing of human A549 cells with APOBEC3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= APOBEC3A antibody.

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CodeSizePrice
NSJ-RQ4184-100ug100 ug£535.00
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Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Flow Cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the APOBEC3A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, also known as APOBEC3A, is a gene of the APOBEC3 family found in humans, non-human primates, and some other mammals.This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids M1-L63 was used as the immunogen for the APOBEC3A antibody.
Limitation:
This APOBEC3A antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P31941