NSJ Bioreagents

Bcl2L2 Antibody

Product Code:
 
NSJ-R30497
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Bcl2L2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
Western blot testing of human HeLa cell lysate with Bcl2L2 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
2 / 4
Western blot testing of human 1) A549, 2) K562, 3) U937, 4) HeLa and 5) A431 cell lysate with Bcl2L2 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
3 / 4
IHC testing of FFPE human rectal cancer tissue with Bcl2L2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human PC-3 cells with Bcl2L2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bcl2L2 antibody.
❮❯

Western blot testing of human HeLa cell lysate with Bcl2L2 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
Western blot testing of human 1) A549, 2) K562, 3) U937, 4) HeLa and 5) A431 cell lysate with Bcl2L2 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
IHC testing of FFPE human rectal cancer tissue with Bcl2L2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human PC-3 cells with Bcl2L2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bcl2L2 antibody.

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CodeSizePrice
NSJ-R30497-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Bcl2L2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Bcl-2-like protein 2 is a protein that in humans is encoded by the BCL2L2 gene. This gene encodes a pro-survival (anti-apoptotic) member of the bcl-2 protein family. Its is mapped to human chromosome 14q11.2-q12. The protein can be found in brain, colon and salivary gland. Acting as an anti-apoptotic/pro-survival regulator, it has played a vital role in reducing cell apoptosis under cytotoxic conditions.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human Bcl2L2 (SAQQRFTQVSDELFQ) was used as the immunogen for this Bcl2L2 antibody (100% homologous in human, mouse and rat).
Limitation:
This Bcl2L2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q92843