NSJ Bioreagents

CD105 Antibody / Endoglin

Product Code:
 
NSJ-R32935
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CD105 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 7
IHC testing of FFPE mouse kidney tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
2 / 7
IHC testing of FFPE mouse liver tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 7
IHC testing of FFPE mouse spleen tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 7
IHC testing of FFPE rat kidney tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 7
IHC testing of FFPE rat liver tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
6 / 7
IHC testing of FFPE rat spleen tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
7 / 7
Western blot testing of mouse lung tissue lysate with CD105 antibody at 0.5ug/ml. Expected molecular weight: 70/90 kDa (monomer, unmodified/glycosylated); 140-180 kDa (dimer, unmodified/glycosylated).
❮❯

IHC testing of FFPE mouse kidney tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse liver tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse spleen tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat kidney tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat liver tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat spleen tissue with CD105 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of mouse lung tissue lysate with CD105 antibody at 0.5ug/ml. Expected molecular weight: 70/90 kDa (monomer, unmodified/glycosylated); 140-180 kDa (dimer, unmodified/glycosylated).

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CodeSizePrice
NSJ-R32935-100ug100 ug£535.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA (recombinant mouse protein): 0.1-0.5ug/ml (BSA-free format available)
Application Note:
Optimal dilution of the CD105 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Endoglin (Osler-Rendu-Weber syndrome 1), CD105, is a type I membrane glycoprotein located on cell surfaces and is a part of the TGF beta receptor complex. Its gene is mapped to human chromosome 8. The protein consists of a homodimer of 180 kDA with disulfide links. It has been found on endothelial cells, activated macrophages, fibroblasts and smooth muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling and has been found to be elevated in pregnant women who subsequently develop preeclampsia.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse protein corresponding to amino acids E27-L226 was used as the immunogen for the CD105 antibody.
Limitation:
This CD105 antibody is available for research use only.
Localization:
Membrane
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
Q63961