NSJ Bioreagents

CD163 Antibody

Product Code:
 
NSJ-R30974
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the CD163 antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 3
IHC staining of FFPE human lung cancer tissue with CD163 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human liver cancer tissue with CD163 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of human HCCT cell lysate with CD163 antibody. Predicted molecular weight ~130 kDa but may be observed at higher molecular weights due to glycosylation.
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IHC staining of FFPE human lung cancer tissue with CD163 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with CD163 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human HCCT cell lysate with CD163 antibody. Predicted molecular weight ~130 kDa but may be observed at higher molecular weights due to glycosylation.

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CodeSizePrice
NSJ-R30974-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the CD163 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cluster of Differentiation 163, also known as Hemoglobin scavenger receptor, is a human protein encoded by the CD163 gene. The receptor belongs to the scavenger receptor cysteine rich family type B and consists of an 1048 amino acid residues extracellular domain, a single transmembrane segment and a cytoplasmic tail with several splice variants. CD163 is a scavenger receptorfor the hemoglobin-haptoglobin complex. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, Stover et al.(2000) mapped the gene to chromosome 12p13.3. CD163 expression in monocytes promoted bacteria-induced proinflammatory cytokine production that could be blocked by CD163 antibody. Cells expressing human CD163 and recombinant protein containing the extracellular domain of CD163 supported adhesion of erythroblastic cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human partial partial recombinant protein (amino acids 1056-1165) was used as the immunogen for this CD163 antibody.
Limitation:
This CD163 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q86VB7