NSJ Bioreagents

Cytokeratin 8 Antibody

Product Code:
 
NSJ-R30341
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the Cytokeratin 8 antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 3
IHC staining of FFPE human intestinal cancer tissue with Cytokeratin 8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) human MCF-7, 2) human Caco-2, 3) human HepG2, 4) human Jurkat, 5) rat RH35 and 6) mouse NIH 3T3 cell lysate with Cytokeratin 8 antibody. Predicted molecular weight ~54 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human Caco-2 cells with Cytokeratin 8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytokeratin 8 antibody.
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IHC staining of FFPE human intestinal cancer tissue with Cytokeratin 8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human MCF-7, 2) human Caco-2, 3) human HepG2, 4) human Jurkat, 5) rat RH35 and 6) mouse NIH 3T3 cell lysate with Cytokeratin 8 antibody. Predicted molecular weight ~54 kDa.
Flow cytometry testing of fixed and permeabilized human Caco-2 cells with Cytokeratin 8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cytokeratin 8 antibody.

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CodeSizePrice
NSJ-R30341-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Cytokeratin 8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cytokeratin 8(CK8), also known as Keratin 8(K8), is a type II keratin. Among the various intermediate filament(IF) proteins, cytokeratin 8(CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells. Cytokeratin 8 mRNA was abundant in fetal small intestine, placenta, pancreas, lung, liver, and kidney. Levels of cytokeratin 8 mRNA in placenta increased slightly during pregnancy. He et al.(2002) presented evidence that K8 is a new cytoplasmic target for JNK in Fas receptor-mediated signaling. The functional significance of this phosphorylation could relate to regulation of JNK signaling and/or regulation of keratin dynamics.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human Cytokeratin 8 (QQQKTARSNMDNMFESYIN) was used as the immunogen for this Cytokeratin 8 antibody.
Limitation:
This Cytokeratin 8 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P05787