NSJ Bioreagents

DJ-1 Antibody

Product Code:
 
NSJ-R31721
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the DJ-1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
Western blot testing of DJ-1 antibody and human samples 1: PANC; 2: U20S; 3: SMMC-7721; 4: HeLa lysate. Predicted/observed size ~20KD
2 / 5
IHC-P: DJ-1 antibody testing of human pancreatic cancer tissue. HIER: steamed with pH6 citrate buffer.
3 / 5
IHC-P: DJ-1 antibody testing of mouse pancreas tissue. HIER: steamed with pH6 citrate buffer.
4 / 5
IHC-P: DJ-1 antibody testing of rat pancreas tissue. HIER: steamed with pH6 citrate buffer.
5 / 5
ICC testing of human A549 cells with DJ-1 antibody.
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Western blot testing of DJ-1 antibody and human samples 1: PANC; 2: U20S; 3: SMMC-7721; 4: HeLa lysate. Predicted/observed size ~20KD
IHC-P: DJ-1 antibody testing of human pancreatic cancer tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: DJ-1 antibody testing of mouse pancreas tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: DJ-1 antibody testing of rat pancreas tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of human A549 cells with DJ-1 antibody.

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CodeSizePrice
NSJ-R31721-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the DJ-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Parkinson disease 7, also known as Protein deglycase DJ-1, is a protein which in humans is encoded by the PARK7 gene. PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It is also involved in tumorigenesis and in maintaining mitochondrial homeostasis. DJ-1 may function as a redox-sensitive chaperone, as a sensor foroxidative stress, and it apparently protects neurons against oxidative stress and cell death. It has been found that DJ-1 mutations that impair transcriptional coactivator function can render dopaminergic neurons vulnerable to apoptosis and may contribute to the pathogenesis of Parkinson disease.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
11315
Immunogen:
Human partial recombinant protein (AA 2-189) was used as the immunogen for this DJ-1 antibody.
Limitation:
DJ-1 antibody is available for research use only.
Localization:
Cytoplasmic & Nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat