NSJ Bioreagents

E2F6 Antibody

Product Code:
 
NSJ-R30909
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the E2F6 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of E2F6 antibody and Lane 1: HeLa; 2: COLO320; 3: A549; 4: MCF-7; 5: SMMC-7721 cell lysate
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Western blot testing of E2F6 antibody and Lane 1: HeLa; 2: COLO320; 3: A549; 4: MCF-7; 5: SMMC-7721 cell lysate

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NSJ-R30909-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the E2F6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
E2F transcription factor 6 is also known as E2F-6. Northern blot analysis revealed that E2F6 is expressed as two mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse protein sequences are 92% identical. The DNA-binding and dimerization domains of E2F6 are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6 can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. It contributes to gene silencing in a manner independent of retinoblastoma protein family members. It is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites.The E2F6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human E2F6 (KDCAQQLFELTDDKEN) was used as the immunogen for this E2F6 antibody.
Limitation:
This E2F6 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O75461