NSJ Bioreagents

EIF3e Antibody

Product Code:
 
NSJ-R32741
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the EIF3e antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of 1) rat thymus, 2) mouse thymus, 3) mouse spleen, 4) human COLO320 and 5) human HeLa lysate with EIF3e antibody at 0.5ug/ml. Expected molecular weight: 48-52 kDa.
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Western blot testing of 1) rat thymus, 2) mouse thymus, 3) mouse spleen, 4) human COLO320 and 5) human HeLa lysate with EIF3e antibody at 0.5ug/ml. Expected molecular weight: 48-52 kDa.

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NSJ-R32741-100ug100ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
Optimal dilution of the EIF3e antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids A160-Q241 from the human protein were used as the immunogen for the EIF3e antibody.
Limitation:
This EIF3e antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P60228