NSJ Bioreagents

GAP43 Antibody

Product Code:
 
NSJ-R30037
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
GAP-7B10, aka GAP-8A12
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the GAP43 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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IHC testing of FFPE rat heart tissue with GAP43 antibody. HIER: steamed with pH6 citrate buffer, HRP-staining.
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IHC testing of FFPE rat heart tissue with GAP43 antibody. HIER: steamed with pH6 citrate buffer, HRP-staining.

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NSJ-R30037-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,IHC (Paraffin): 1-2ug/ml,Immunohistochemistry (Frozen): 1-2ug/ml--formalin or acetone fixed tissues
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the GAP43 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
2596
Immunogen:
GAP-43 from neonatal rat forebrain membranes was used as the immunogen for this GAP43 antibody.
Limitation:
This GAP43 antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, mouse, rat