NSJ Bioreagents

GFAP Antibody

Product Code:
 
NSJ-R30340
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the GFAP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain
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IHC-P: GFAP antibody testing of rat brain tissue
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Western blot testing of GFAP antibody and Lane 1. rat brain, 2: mouse brain tissue lysate
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Immunofluorescent staining of mouse brain tissue with GFAP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain
IHC-P: GFAP antibody testing of rat brain tissue
Western blot testing of GFAP antibody and Lane 1. rat brain, 2: mouse brain tissue lysate
Immunofluorescent staining of mouse brain tissue with GFAP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-R30340-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the GFAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glial fibrillary acidic protein is an intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. Expression is also seen in rat kidney glomeruli and peritubular fibroblasts, leydig cells of testis, skin keratinocytes, osteocytes of bones, chondrocytes of epiglottis, bronchus, and stellate-shaped cells of the pancreas and liver. Its expression is essential for normal white matter architecture and blood-brain barrier integrity, and its absence leads to late-onset CNS dysmyelination. The protein has also been shown to play a role in mitosis by adjusting the filament network present in the cell. During mitosis, there is an increase in the amount of phosphorylated GFAP, and a movement of this modified protein to the cleavage furrow.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human GFAP (DGEVIKESKQEHKDVM) was used as the immunogen for this GFAP antibody (100% homologous in human and rat).
Limitation:
This GFAP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14136