NSJ Bioreagents

GPR30 Antibody

Product Code:
 
NSJ-R31359
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the GPR30 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of GPR30 antibody and Lane 1: human COLO320; 2: (h) MCF-7; 3: monkey COS7 cell lysate. Expected size (unglycosylated) ~40KD, glycosylated size ~60KD
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Western blot testing of GPR30 antibody and Lane 1: human COLO320; 2: (h) MCF-7; 3: monkey COS7 cell lysate. Expected size (unglycosylated) ~40KD, glycosylated size ~60KD

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CodeSizePrice
NSJ-R31359-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the GPR30 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
G-protein coupled estrogen receptor 1 (GPER1), also known as the GPR30 or CEPR, is a G protein-coupled receptor that in humans is encode d by the GPER gene. This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. GPR30 therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human G-protein coupled receptor 30 (ENVFISVHLLQRTQ) was used as the immunogen for this GPR30 antibody.
Limitation:
This GPR30 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Monkey
Uniprot #:
Q99527