NSJ Bioreagents

GPX1 Antibody

Product Code:
 
NSJ-R31616
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the GPX1 antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 3
IHC staining of FFPE human colorectal adenocarcinoma tissue with GPX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE rat lung tissue with GPX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing 1) human ThP-1, 2) rat lung, 3) rat liver, 4) mouse lung and 5) mouse liver lysate with GPX1 antibody. Predicted molecular weight ~22 kDa.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with GPX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lung tissue with GPX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing 1) human ThP-1, 2) rat lung, 3) rat liver, 4) mouse lung and 5) mouse liver lysate with GPX1 antibody. Predicted molecular weight ~22 kDa.

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NSJ-R31616-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the GPX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glutathione peroxidase 1, also known as, GPX-1 is an enzyme that in humans is encoded by the GPX1 gene. It is mapped to 3p21.31. This gene encodes a member of the glutathione peroxidase family consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. GPX1 is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
2876
Immunogen:
An amino acid sequence from the middle region of human Glutathione peroxidase 1 (EVNGAGAHPLFAFLREALPAPSDDATALMTD) was used as the immunogen for this GPX1 antibody.
Limitation:
This GPX1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat