NSJ Bioreagents

IL-18 Antibody

Product Code:
 
NSJ-R31447
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution the IL-18 antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE rat spleen tissue with IL-18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE mouse spleen tissue with IL-18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of mouse spleen tissue lysate with IL-18 antibody. Predicted molecular weight: 17~24 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized mouse HEPA1-6 cells with IL-18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL-18 antibody.
❮❯

IHC staining of FFPE rat spleen tissue with IL-18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse spleen tissue with IL-18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse spleen tissue lysate with IL-18 antibody. Predicted molecular weight: 17~24 kDa.
Flow cytometry testing of fixed and permeabilized mouse HEPA1-6 cells with IL-18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL-18 antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-R31447-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the IL-18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Interleukin-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL-18 is a cytokine that modulates both T helper type 1 (Th1) and Th2 responses. It is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
29197
Immunogen:
Rat partial recombinant protein (AA 37-194) was used as the immunogen for this IL-18 antibody.
Limitation:
This IL-18 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Rat, Mouse