NSJ Bioreagents

MKK7 Antibody (MEK7)

Product Code:
 
NSJ-R31022
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the MKK7 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of 1) human HeLa, 2) monkey COS-7, 3) rat skeletal muscle and 4) mouse skeletal muscle tissue lysate with MKK7 antibody. Predicted molecular weight ~47 kDa.
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Western blot testing of 1) human HeLa, 2) monkey COS-7, 3) rat skeletal muscle and 4) mouse skeletal muscle tissue lysate with MKK7 antibody. Predicted molecular weight ~47 kDa.

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NSJ-R31022-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MKK7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MAP2K7 (Mitogen-activated protein kinase kinase 7), also known as MAP kinase kinase 7 (MAPKK7), JNKK2, PRKMK7, Mek7 and MKK7, is an enzyme that in humans is encoded by the MAP2K7 gene. This protein is a member of the mitogen-activated protein kinase kinase family. The protein exists as six different isoforms with three possible N-termini (alpha, beta, and gamma isoforms) and two possible C-termini (1 and 2 isoforms). Schramek et al.(2011) showed that the doxorubicin-mediated DNA damage response in human A549 lung carcinoma cells caused rapid phosphorylation and upregulation of p53 (TP53). MKK7 / Mek7 knockdown reduced p53 phosphorylation, delayed p53 upregulation, and interfered with cell cycle arrest at G2/M. the protein was activated in primary lung tumors, and tumors with a p53 mutation showed even higher MKK7 phosphorylation. Schramek et al.(2011) concluded that MKK7 / Mek7 exerts its tumor suppressive function through p53.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human MEK7/MKK7 (ARRRIDLNLDISPQRPR) was used as the immunogen for this MKK7 antibody (100% homologous in human, mouse and rat).
Limitation:
This MKK7 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O14733