NSJ Bioreagents

MMP16 Antibody

Product Code:
 
NSJ-R32966
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the MMP16 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 7
Immunofluorescent staining of FFPE human A431 cells with MMP16 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
IHC testing of FFPE human lung cancer tissue with MMP16 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 7
IHC testing of FFPE human breast cancer tissue with MMP16 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 7
IHC testing of FFPE human colon cancer tissue with MMP16 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 7
IHC testing of FFPE human placental tissue with MMP16 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
6 / 7
Western blot testing of 1) mouse intestine and 2) human SMMC-7721 lysate with MMP16 antibody at 0.5ug/ml. Predicted molecular weight: ~70 kDa (long form), ~53 kDa (short form).
7 / 7
Flow cytometry testing of human Caco-2 cells with MMP16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MMP16 antibody.
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Immunofluorescent staining of FFPE human A431 cells with MMP16 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human lung cancer tissue with MMP16 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human breast cancer tissue with MMP16 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human colon cancer tissue with MMP16 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human placental tissue with MMP16 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of 1) mouse intestine and 2) human SMMC-7721 lysate with MMP16 antibody at 0.5ug/ml. Predicted molecular weight: ~70 kDa (long form), ~53 kDa (short form).
Flow cytometry testing of human Caco-2 cells with MMP16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MMP16 antibody.

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CodeSizePrice
NSJ-R32966-100ug100 ug£535.00
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Further Information

Application Details :
Western Blot: 0.5-1ug/ml,IHC (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA (recombinant human protein): 0.1-0.5ug/ml (BSA-free format available)
Application Note:
Optimal dilution of the MMP16 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
The Matrix metalloproteinase 16 (MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14, MMP15, and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids Y120-I296 was used as the immunogen for the MMP16 antibody.
Limitation:
This MMP16 antibody is available for research use only.
Localization:
Cytoplasmic, membrane, secreted
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P51512