NSJ Bioreagents

Tissue Factor Antibody / CD142

Product Code:
 
NSJ-R31962
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the Tissue Factor antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human A431 cells with Tissue Factor antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of human 1) A431 and 2) HaCaT cell lysate with Tissue Factor antibody. Expected molecular weight: 33-50 kDa depending on glycosylation level.
3 / 4
Flow cytometry testing of human A431 cells with Tissue Factor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tissue Factor antibody.
4 / 4
IHC staining of FFPE human placental tissue with Tissue Factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human A431 cells with Tissue Factor antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A431 and 2) HaCaT cell lysate with Tissue Factor antibody. Expected molecular weight: 33-50 kDa depending on glycosylation level.
Flow cytometry testing of human A431 cells with Tissue Factor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tissue Factor antibody.
IHC staining of FFPE human placental tissue with Tissue Factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-R31962-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note:
Optimal dilution of the Tissue Factor antibody should be determined by the researcher.
Description:
Tissue factor also called platelet tissue factor, factor III, or CD142. The F3 gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 33-295 of human TF/Factor III were used as the immunogen for the Tissue Factor antibody.
Limitation:
This Tissue Factor antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P13726