NSJ Bioreagents

TJP2 Antibody (ZO-2)

Product Code:
 
NSJ-R31077
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the TJP2 antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE mouse liver tissue with TJP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat liver tissue with TJP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with TJP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 4
Western blot testing of 1) human RT4, 2) human A431, 3) human HepG2, 4) human MCF7, 5) human U-2 OS, 6) human HeLa, 7) human PC-3, 8) rat RH35 and 9) mouse HEPA1-6 cell lysate with TJP2 antibody. Expected molecular weight: 131-160 kDa.
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IHC staining of FFPE mouse liver tissue with TJP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with TJP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with TJP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human RT4, 2) human A431, 3) human HepG2, 4) human MCF7, 5) human U-2 OS, 6) human HeLa, 7) human PC-3, 8) rat RH35 and 9) mouse HEPA1-6 cell lysate with TJP2 antibody. Expected molecular weight: 131-160 kDa.

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NSJ-R31077-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the TJP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Tight Junction Protein 2, also known as Zona Occludens 2 or ZO-2, is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of mouse Tight Junction Protein 2 (AHSKRGYYSQPSRYRDTEL) was used as the immunogen for this TJP2 antibody (100% rat homology).
Limitation:
This TJP2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
Q9Z0U1