VDR Antibody (Vitamin D Receptor)

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the VDR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Vitamin D Receptor, also known as Vitamin D Hormone Receptor, is a member of the nuclear receptor family of transcription factors. Labuda et al.(1991) assigned the VDR gene to 12q12-q14 by in situ hybridization. Using mutation analysis, Jurutka et al.(2000) characterized arg18/arg22, VDR residues immediately N-terminal of the first DNA-binding zinc finger, as vital for contact with the general transcription factor IIB(TFIIB). A natural polymorphic variant of the receptor, termed F/M4(missing a FokI restriction site), which lacks only the first 3 amino acids(including glu2), interacted more efficiently with TFIIB and also possessed elevated transcriptional activity compared with the full-length(f/M1) receptor. Shah et al.(2006) stated that the signaling and oncogenic activity of beta-catenin(CTNNB1) can be repressed by activation of VDR. Conversely, high levels of beta-catenin can potentiate the transcriptional activity of 1,25-dihydroxyvitamin D3.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the C-terminus of human Vitamin D Receptor (DLRSLNEEHSKQYRCL) was used as the immunogen for this VDR antibody.

This VDR antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

P11473