NSJ Bioreagents

VEGFR2 Antibody

Product Code:
 
NSJ-R31095
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the VEGFR2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
Western blot testing with VEGFR2 antibody and SMMC-7721 lysate
2 / 4
IHC-P: VEGFR2 antibody testing of human lung cancer tissue
3 / 4
ICC testing of VEGFR2 antibody and HeLa cells
4 / 4
IHC-F testing of VEGFR2 antibody and human placenta tissue
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Western blot testing with VEGFR2 antibody and SMMC-7721 lysate
IHC-P: VEGFR2 antibody testing of human lung cancer tissue
ICC testing of VEGFR2 antibody and HeLa cells
IHC-F testing of VEGFR2 antibody and human placenta tissue

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CodeSizePrice
NSJ-R31095-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the VEGFR2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
KDR(Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al.(1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor(VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1(Flk1) in mouse, is exclusively expressed in endothelial cells(Plate et al., 1993). Flk1 is the mouse homolog of KDR(Matthews et al., 1991).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human VEGF Receptor 2 (HHIHWYWQLEEECANE) was used as the immunogen for this VEGFR2 antibody.
Limitation:
This VEGFR2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P35968